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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which ends up in chromosome instability, and therefore may contribute to tumorigenesis. This protein was found to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential position in advertising and marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already described. Two additional splice variants have been noted but in-depth nucleotide sequence data is not nonetheless offered.
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Blocking peptides are peptides that bind specially to your goal antibody and block antibody binding. These peptide usually has the epitope acknowledged through the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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